relationship between BRCA1 mRNA expression
The journal outlines a study that was based on previous studies done to examine the role of Breast Cancer Susceptibility Gene in regulating chemotherapy induced damage of the DNA considering its fundamental component that is involved in varied aspects pertaining to responses to DNA damage, as well as the pivotal role pertaining to DNA repair pathway. The study had the sole objective of determining whether there existed any relationship between BRCA1 mRNA expression levels as prognostic and predictive biomarkers and clinical outcomes pertaining to patients with esophageal cancer who had received treatment based on docetaxel or cisplatin (Gao et al, 2013). In this study, 155 patients who had histologically confirmed metastatic or locally advanced esophageal squamous cell carcinoma, as well as available paraffin embedded tumor material to be used in molecular analysis were incorporated in this study.
The study involved the examination of BRCA1 gene expression in paraffin-embedded specimens through biopsy endoscope derived from the patients. The researchers also carried out quantitative real-time polymerese chain reaction so as to quantify the gene expression by the use of ABI Prism 7900HT Sequence Detection System (Gao et al, 2013). The technique is recognized as more accurate and quantitative than immunohistochemistry in evaluating protein expression as a biomarker.
The researchers successfully detected BRCA1 mRNA expression in 144 tumor samples through the utilization of quantitative real-time PCR. The study established no significant correlation between expression levels of BRCA1 and clinical characteristics such as gender, age, stage, performance status and site of tumor. There is a correlation between Low BRCA1 mRNA expression and increased response rate and median overall survival in chemoradiotherapy and cisplatin-based therapy, as well as decreased RR and mOS in chemoradiotherapy or docetaxel-based group (Gao et al, 2013). In addition, Multivariate analysis showed low BRCA1 expression to be an independent prognostic factor in chemoradiotherapy or cisplatin-based chemotherapy group, as well as a higher risk for mortality in chemoradiotherapy or docetaxel-based chemotherapy group (Gao et al, 2013).
The key strengths of the study lies in the comprehensive nature of the information provided. It offers comprehensive information about previous studies done that formed a basis for the study. However, its weakness lies in the inability of establishing a link between the previous studies and this one. It is also noted that the study used relatively small number of participants, in the treatment subgroups.
Gao, Y., Zhu, J., Zhang, X., Wu, Q., Jiang, S., Liu, Y., Hu, Z., Liu, B., Chen, X, 2013. BRCA1 mRNA Expression as a Predictive and Prognostic Marker in Advanced Esophageal Squamous Cell Carcinoma Treated with Cisplatin- or Docetaxel-Based Chemotherapy/Chemoradiotherapy. PLOSE. Vol. 8, Issue 1
Are you busy and do not have time to handle your assignment? Are you scared that your paper will not make the grade? Do you have responsibilities that may hinder you from turning in your assignment on time? Are you tired and can barely handle your assignment? Are your grades inconsistent?
Whichever your reason may is, it is valid! You can get professional academic help from our service at affordable rates. We have a team of professional academic writers who can handle all your assignments.
Our essay writers are graduates with diplomas, bachelor's, masters, Ph.D., and doctorate degrees in various subjects. The minimum requirement to be an essay writer with our essay writing service is to have a college diploma. When assigning your order, we match the paper subject with the area of specialization of the writer.
You fill all the paper instructions in the order form. Make sure you include all the helpful materials so that our academic writers can deliver the perfect paper. It will also help to eliminate unnecessary revisions.
Proceed to pay for the paper so that it can be assigned to one of our expert academic writers. The paper subject is matched with the writer’s area of specialization.
You communicate with the writer and know about the progress of the paper. The client can ask the writer for drafts of the paper. The client can upload extra material and include additional instructions from the lecturer. Receive a paper.
The paper is sent to your email and uploaded to your personal account. You also get a plagiarism report attached to your paper.
PLACE THIS ORDER OR A SIMILAR ORDER WITH ESSAY OVEN TODAY AND GET AN AMAZING DISCOUNT